Rai et al (2014) wished to discover the differences in MTHFR prevalence in normal and colon cancer tissue. Therefore, DNA isolated from tumour tissue was examined and compared with normal tissue of colon cancer patients. Results found the frequencies of MTHFR C677T and A1298C were significantly different between tumour and normal tissue in colon cancer patients. Overall, the A1298C mutation was found to be associated with colon cancer. The authors concluded that MTHFR variations may exists within difference tissues in order to maintain normal function, but then may have implications for susceptibility to disease.